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Isolation of brucella spp from milk of brucellosis positive cows in São Paulo and Minas Gerais states Braz. J. Vet. Res. Anim. Sci.
LANGONI,Helio; ICHIHARA,Sílvio Massaru; SILVA,Aristeu Vieira da; PARDO,Renata Bonini; TONIN,Flávia Bechelli; MENDONÇA,Lia Jeanne Pereira; MACHADO,José Aparecido Dorta.
Brucellosis is a chronic zoonosis that plays an important role in Public Health. Considering the lack of data in Brazil regarding its presence in raw milk and non-pasteurized dairy products, we determined the presence of brucellae in milk from brucellosis positive animals. The slide agglutination test (SAT), tube agglutination test (TAT) and TAT treated with 2-mercaptoethanol were used to identify positive animals in studied herds. For 3 days, 300 ml milk samples/cow (75 ml/teat) were collected from all productive quarters of the positive animals. These were mixed and centrifuged. Part of the pellet and of the supernatant were inoculated in Farrel and Brodie-Sinton (BS) media supplemented with antimicrobial agents. The inoculated plates and tubes were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Milk; Brucella abortus; Cows; Brucellosis.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000600004
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